Calnexin antibody (C-Term, Intracellular)

Catalog No. ABIN7884841

The Rabbit Polyclonal anti-Calnexin antibody is suitable to detect Calnexin in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for Calnexin antibody (C-Term, Intracellular) (ABIN7884841)

Target: Calnexin (CANX)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Calnexin Antibody

Binding Specificity: AA 572-587, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Calnexin

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx

Isotype: IgG

Application Details

Application Notes:

WB: 1:400-1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235025)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Canx

Background:

Synonyms: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

Description: Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a -RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of Shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.Coexpression of calnexin with the voltage-gated potassium channel KV1.2α was found to produce a substantial dose-dependent increase in cell surface KV1.2α channels in transfected mammalian COS-1 cells. In contrast, calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6α subunits3.Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.

Gene ID: 29144

UniProt: P35565

Pathways: MAPK Signaling, Thyroid Hormone Synthesis