CD97 antibody (Extracellular, N-Term)

Catalog No. ABIN7884854

The Rabbit Polyclonal anti-CD97 antibody (ABIN7884854) specifically detects CD97 in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for CD97 antibody (Extracellular, N-Term) (ABIN7884854)

Target: CD97

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD97 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-CD97 Antibody

Binding Specificity: AA 499-512, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to CD97/ADGRE5

Predicted Reactivity: Human only

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KSDSDRGGHWATEG, corresponding to amino acid residues 499-512 of human CD97/ADGRE5

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CD97

Target: CD97

Alternative Name: Adhesion G protein-coupled receptor E5

Background:

Synonyms: Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation

Description: CD97, encoded by the adhesion G protein-coupled receptor 5 (ADGRE5) gene, is a member of the epidermal growth factor (EGF) seven transmembrane receptor family, belonging to the adhesion family of G protein-coupled receptors (GPCRs)1. CD97 is widely expressed on the surface of lymphoid cells, macrophages, smooth muscle cells and several types of tumor cells and acts through binding of decay accelerating factor (CD55) and dermatan sulfate2.In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation3. Antibodies against CD97 have been demonstrated to diminish various inflammatory disorders by depleting granulocytes and the interaction between CD97 and its ligand CD55 regulates T-cell activation and increases proliferation and cytokine production4.Changes in the expression of CD97 have been described in auto-inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Outside the immune system, CD97 is likely involved in cell-cell interactions and ectopic CD97 expression leading to enhanced cell-cell aggregation5.

Gene ID: 976

UniProt: P48960