Cortactin antibody (Intracellular)

Catalog No. ABIN7884899

The Rabbit Polyclonal anti-Cortactin antibody (ABIN7884899) specifically detects Cortactin in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cortactin antibody (Intracellular) (ABIN7884899)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-Cortactin Antibody

Binding Specificity: AA 346-359, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Cortactin

Predicted Reactivity: Rat,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235169)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Src substrate cortactin

Background:

Synonyms: Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN

Description: Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63-65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.

Gene ID: 13043

UniProt: Q60598

Pathways: MAPK Signaling