ENPP1 antibody (C-Term, Extracellular)

Catalog No. ABIN7884924

This Rabbit Polyclonal antibody specifically detects ENPP1 in WB, IHC, FACS and LCI. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ENPP1 antibody (C-Term, Extracellular) (ABIN7884924)

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENPP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-ENPP1 Antibody

Binding Specificity: AA 711-725, C-Term, Extracellular

Purpose: A Rabbit Polyclonal antibody to ENPP1 (extracellular)

Predicted Reactivity: Mouse - 14 out of 15 amino acid residues identical, Rat- 13 out of 15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: CLYQDFRIPLSPVHK, corresponding to amino acid residues 711 - 725 of human ENPP1 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ENPP1

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Alternative Name: Ectonucleotide Pyrophosphatase/ Phosphodiesterase Family Member 1

Background:

Synonyms: Ectonucleotide Pyrophosphatase/ Phosphodiesterase Family Member 1, Plasma-Cell Membrane Glycoprotein PC-1, CD203a, Membrane Component Chromosome 6 Surface Marker 1, M6S1

Description: Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), also known as Plasma-Cell Membrane Glycoprotein (PC-1) and CD203a, is a member the ecto-enzyme ENPP family that includes seven members (ENPP1-ENPP7)1.ENPP1 is involved in purinergic signaling as it catalyzes the hydrolysis of ATP or GTP to AMP or GMP, while generating inorganic pyrophosphates (PPi)1,2. Inorganic pyrophosphates inhibit bone and cartilage mineralization, hence making ENPP1 a key regulator of bone and cartilage development and remodeling 2,3. Indeed, several hereditary mineralization or calcification-related disorders, have been linked to loss-of-function mutations of ENPP1, such as general arterial calcification of infancy (GACI) or Cole disease 3.Recently, an intriguing new role of ENPP1 as a central regulator of immunity and tumor proliferation came to light. It was shown that ENPP1 is the critical enzyme that degrades the stimulator of interferon genes (STING) ligand, cyclic GMP-AMP (cGAMP)4,5. cGAMP was first described as an intracellular second messenger, synthesized by the enzyme cyclic-GMP-AMP synthase (cGAS) in response to self and pathogenic double-stranded DNA in the cytosol. Intracellular signaling of cGAMP is carried through the STING pathway which then directs several antiviral and anticancer cellular response mechanisms 4,5.cGAMP can also be released to the extracellular space where it can be transported into cells by receptors like MERTK 6, P2X7 6, SLC19A1 7 and LRRC8A 8. ENPP1 is the only known ecto-enzyme that can inactivate extracellular cGAMP to GMP and AMP, and thus functions as an inhibitor of the anti-tumor response elicited by extracellular cGAMP 4,9.

Gene ID: 5167

UniProt: P22413

Pathways: Regulation of Carbohydrate Metabolic Process