EPH Receptor A2 antibody (Extracellular, N-Term)

Catalog No. ABIN7884934

The Rabbit Polyclonal anti-EPH Receptor A2 antibody is suitable to detect EPH Receptor A2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS and LCI.

Quick Overview for EPH Receptor A2 antibody (Extracellular, N-Term) (ABIN7884934)

Target: EPH Receptor A2 (EPHA2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-EPH Receptor A2 Antibody

Binding Specificity: AA 333-347, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to EphA2 Receptor

Predicted Reactivity: Rat - 14,15 amino acid residues identical, mouse - 13

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HYLTAVGMGAKVELR, corresponding to amino acid residues 333 - 347 of human EphA2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235278)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: Ephrin type A receptor 2

Background:

Synonyms: Ephrin type A receptor 2, EPH Receptor A2, Epithelial Cell Kinase, Tyrosine-Protein Kinase Receptor ECK, ECK, EPHA2

Description: Erythropoietin-producing hepatocellular receptor A2 (EphA2) is a tyrosine kinase that belongs to the Ephrin (Eph) family of receptors. Members bind to cell surface-associated Ephrin ligands on neighboring cells. This binding generates bidirectional signaling which is a major form of contact-dependent communication between cells1. Eph receptors are divided into two classes, EphA and EphB, depending on the homology of their extracellular domains. Each extracellular domain is composed of a ligand-binding domain, Sushi domain, epidermal growth factor (EGF)-like domain, and two fibronectin type-III repeats. Eph receptors also contain a transmembrane domain, juxtamembrane region, tyrosine kinase domain, sterile alpha motif (SAM) and postsynaptic density (PSD)-95 protein, Discs large, and Zona occludens tight junction protein (PDZ) domain-binding motif.The interaction between Ephrin and the Eph receptor results in both "forward signaling" and "reverse signaling," which are mediated by Eph receptor and Ephrin, respectively2. Forward signaling induces Eph receptor oligomer clustering, cross-phosphorylation of tyrosine residues on the juxtamembrane domain and activation loop and, consequently, activation of the kinase activity.Eph receptor-ligand interactions mainly regulate cell proliferation and migration during development as well as tissue homeostasis, axon guidance, synapse plasticity, tissue remodeling, bone morphogenesis, and angiogenesis2.EphA2 is expressed in normal epidermal cells and in various solid tumors, such as breast, ovary, prostate, pancreas, glioblastoma, neck, renal, lung, melanoma, bladder and other types of cancer2.EphA2 expression in tumors is associated with a more aggressive cancer phenotype and correlates with tumor metastasis and poor patient outcome. In contrast, EphA2 also reduces cancer cell motility and proliferation and hence has both pro- and anti-oncogenic roles, depending on the cellular context3.Due to its pivotal role in various diseases, EphA2 is an appealing drug target and research is performed in order to control its activity4.

Gene ID: 1969

UniProt: P29317

Pathways: RTK Signaling