EPH Receptor B1 antibody (Extracellular, N-Term)

Catalog No. ABIN7884940

The Rabbit Polyclonal anti-EPH Receptor B1 antibody is suitable to detect EPH Receptor B1 in samples from Human, Mouse and Rat. It has been validated for WB, IF, FACS, IC and LCI.

Quick Overview for EPH Receptor B1 antibody (Extracellular, N-Term) (ABIN7884940)

Target: EPH Receptor B1 (EPHB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-EPH Receptor B1 Antibody

Binding Specificity: AA 485-497, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to EphB1 Receptor

Predicted Reactivity: Rat,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSQTNTARIDGLR, corresponding to amino acid residues 485-497 of mouse EphB1

Isotype: IgG

Application Details

Application Notes:

WB: 1:800

FC: 1:15

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235294)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for EPH Receptor B1

Target: EPH Receptor B1 (EPHB1)

Alternative Name: Ephrin type-B receptor 1

Background:

Synonyms: Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2

Description: Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer's disease1.

Gene ID: 270190

UniProt: Q8CBF3

Pathways: RTK Signaling