HRH2 antibody (2nd Extracellular Loop)

Catalog No. ABIN7885078

The Rabbit Polyclonal anti-HRH2 antibody is suitable to detect HRH2 in samples from Rat and Mouse. It has been validated for WB, IF, IHC, FACS, IC and LCI.

Quick Overview for HRH2 antibody (2nd Extracellular Loop) (ABIN7885078)

Target: HRH2 (Histamine Receptor H2 (HRH2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-HRH2 Antibody

Binding Specificity: 2nd Extracellular Loop

Purpose: A Rabbit Polyclonal Antibody to Histamine H2 Receptor

Predicted Reactivity: Mouse - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 1:20

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235754)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HRH2

Target: HRH2 (Histamine Receptor H2 (HRH2))

Alternative Name: H2R

Background:

Synonyms: H2R, HH2R, Gastric receptor I, Gastric receptor 1

Description: Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including the brain, gastrointestinal tract and heart.The most studied physiological role of the H2 receptor is its involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia.Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested.

Gene ID: 25461

UniProt: P25102