MICU1 antibody (Intracellular)

Catalog No. ABIN7885145

The Rabbit Polyclonal anti-MICU1 antibody (ABIN7885145) specifically detects MICU1 in WB. The antibody is reactive with Rat and Mouse samples.

Quick Overview for MICU1 antibody (Intracellular) (ABIN7885145)

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICU1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-MICU1 Antibody

Binding Specificity: AA 313-327, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235963)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MICU1

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Alternative Name: Mitochondrial calcium uptake protein 1

Background:

Synonyms: Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS

Description: Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium-red-sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino-terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C-terminus containing two classical EF-hand Ca2+-binding domains2.Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF-hand domains, exerting an inhibitory role on MCU-dependent Ca2+ entry3.RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.

Gene ID: 365567

UniProt: Q6P6Q9