Presenilin 1 antibody (3rd Intracellular Loop)

Catalog No. ABIN7885319

The Rabbit Polyclonal anti-Presenilin 1 antibody (ABIN7885319) specifically detects Presenilin 1 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Presenilin 1 antibody (3rd Intracellular Loop) (ABIN7885319)

Target: Presenilin 1 (PSEN1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Presenilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Presenilin 1 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 345-359

Purpose: A Rabbit Polyclonal Antibody to Presenilin-1

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDSHLGPHRSTPESR, corresponding to amino acid residues 345-359 of rat Psen1

Isotype: IgG

Application Details

Application Notes:

WB: 1:500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:600

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236593)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Presenilin 1

Target: Presenilin 1 (PSEN1)

Alternative Name: PSEN1

Background:

Synonyms: PSEN1, PS-1, Psnl1

Description: Presenilin-1 (PSEN1) is a transmembrane protein encoded by the PS1 gene. The protein is comprised of 9 transmembrane domains. The N- and C-termini of the protein are cytosolic and lumenal respectively. PSEN1, together with three other proteins - nicastrin, presenilin enhancer 2 and anterior pharynx-defective 1 form a protein complex named γ-Secretase. PSEN1 serves as the catalytic subunit of the γ-secretase complex. This complex, along with α- and β-secretases cleaves the amyloid precursor protein (APP). APP is the precursor for β-Amyloid fibrils which are the pathological hallmark of Alzheimer's disease (AD) and mutations in the PSEN1 gene have been implicated in AD pathophysiology. Currently, it remains unclear whether PSEN1 mutations cause disease by a loss of function or a gain of toxic function mechanism1.PS1 mutations causing an overexpression of mutant human PSEN1 also increase the expression of ryanodine receptor 3 in PC12 cells. In addition, PC12 and cortical neuron cells expressing mutant PSEN1 exhibit increased calcium responses to caffeine compared with cells expressing wildtype PSEN1. This enhanced release of calcium is associated with increased cell vulnerability to β-Amyloid and caffeine induced cellular death. It has been hypothesized that PSEN1 and RyR interact directly2.PS1 mutations also enhance inositol triphosphate (IP3)-mediated Ca2+ release in non-excitable and excitable cells. IP3-evoked Ca2+ responses are more than threefold greater in PS1M146V knock-in mice relative to non-transgenic controls. These mutations specifically disrupt intracellular Ca2+ release rather than reduce cytosolic Ca2+ buffering or clearance3.

Gene ID: 29192

UniProt: P97887

Pathways: Notch Signaling, EGFR Signaling Pathway, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport