Serotonin Receptor 2B antibody (Extracellular, N-Term)

Catalog No. ABIN7885391

The Rabbit Polyclonal anti-Serotonin Receptor 2B antibody (ABIN7885391) specifically detects Serotonin Receptor 2B in WB, IHC, FACS and LCI. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Serotonin Receptor 2B antibody (Extracellular, N-Term) (ABIN7885391)

Target: Serotonin Receptor 2B (HTR2B)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Serotonin Receptor 2B Antibody

Binding Specificity: AA 6-20, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B

Predicted Reactivity: Mouse - identical,human - 11,15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KMSEQ STISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236792)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Serotonin Receptor 2B

Target: Serotonin Receptor 2B (HTR2B)

Alternative Name: 5-Hydroxytryptamine receptor 2B

Background:

Synonyms: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor

Description: 5-HT is involved in a variety of physiological and biological processes. In the brain, 5-HT has been found to affect sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. Like other members, they are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2B receptors have been found to be present in both rodent and human tissues, particularly in the cardiovascular system, gastrointestinal tract, bone, and central nervous system2. Only low expression levels are found in the brain and blood. The highest expression levels are found in the stomach fundus3.Over-expression of 5-HT2B receptors in hearts of transgenic mice results in cardiac hypertrophy and decreased ventricular function due to enhanced extracellular matrix (ECM) and remodeling. Likewise, genetic deletion of 5-HT2B receptors was shown to lead to ventricular dilation and incomplete cardiac development5.

Gene ID: 28581

UniProt: P30994

Pathways: JAK-STAT Signaling, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process