Serotonin Receptor 3A antibody (2nd Cytoplasmic Loop)

Catalog No. ABIN7885392

This Rabbit Polyclonal antibody specifically detects Serotonin Receptor 3A in WB, IHC and IF. It exhibits reactivity toward Rat and Mouse.

Quick Overview for Serotonin Receptor 3A antibody (2nd Cytoplasmic Loop) (ABIN7885392)

Target: Serotonin Receptor 3A (HTR3A)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Serotonin Receptor 3A Antibody

Binding Specificity: 2nd Cytoplasmic Loop, AA 342-355

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3A

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine receptor 3A

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Serotonin Receptor 3A

Target: Serotonin Receptor 3A (HTR3A)

Alternative Name: 5-Hydroxytryptamine receptor 3A

Background:

Synonyms: 5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor 3A

Description: 5-Hydroxytryptamine receptor 3A (5-HT3A, HTR3A) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The 5-HT3A subunit is expressed in GABAergic and enkephalinergic neurons in the spinal dorsal horn thereby marking its possible antinociceptive effect4.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Gene ID: 79246

UniProt: P35563

Pathways: Synaptic Membrane