SYNGAP1 antibody (C-Term)

Catalog No. ABIN7885551

This Guinea Pig Polyclonal antibody specifically detects SYNGAP1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SYNGAP1 antibody (C-Term) (ABIN7885551)

Target: SYNGAP1 (Synaptic Ras GTPase Activating Protein 1 (SYNGAP1))

Reactivity: Human, Mouse, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SYNGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SYNGAP1 Antibody

Binding Specificity: AA 1185-1199, C-Term

Purpose: A Guinea Pig Polyclonal Antibody to SynGAP protein

Predicted Reactivity: Bovine - identical, Mouse - 9,14 amino acid residues identical, Dog - 13, Rat - 12,14 amino acid residues identical.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KSMDESRLDRVKEYE, corresponding to amino acid residues 1185-1199 of rat SynGAP

Isotype: IgG

Application Details

Application Notes: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237319)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SYNGAP1

Target: SYNGAP1 (Synaptic Ras GTPase Activating Protein 1 (SYNGAP1))

Alternative Name: SynGAP

Background:

Synonyms: SynGAP1, Synaptic Ras GTPase-activating protein 1, Synaptic Ras-GAP 1, Neuronal RasGAP, p135 SynGAP

Description:

SynGAP1 is a major signaling protein that plays a pivotal role in regulating fundamental molecular changes in dendritic spine synaptic morphological and functional modifications.

SynGAP1 functions as a downstream component of NMDA receptor-associated signaling and of AMPA receptor trafficking to excitatory postsynaptic membrane. SynGAP1 is mainly expressed in the hippocampus and cortex.

SynGAP1 is a cytosolic protein without a signal peptide or any transmembrane domain. The N-terminal half of SYNGAP1 has a Ras-GAP domain, along with the region that is loosely homologous to Pleckstrin homology and a C2 domain which is potentially involved in binding Ca2+ and phospholipids. Interestingly, the alignment of GAP domain of SynGAP1 with other Ras-GAPs suggests that the amino acids in the GAP domain are crucial for interacting with Ras and for stimulating Ras-GTPase activity. The C-terminal half consists of a repeat of 10 histidines that may be involved in metal chelation, several potential serine and tyrosine phosphorylation sites and a T/SXV motif necessary for interaction with SAP102 and PSD-95.

SynGAP1 has been implicated in the pathogenesis of intellectual disability. de novo out-of-frame deletions were identified in patients with intellectual disability presenting with microcephaly and generalized epilepsy while de novo microdeletions in chromosome region 6p21.3 were observed in patients with severe language impairment in addition to epilepsy. SynGAP1 may also be involved in the pathogenesis of schizophrenia through altered regulation of the NMDA receptor complex.

Gene ID: 192117

UniProt: Q9QUH6

Pathways: Regulation of long-term Neuronal Synaptic Plasticity