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PLK1 antibody (pSer137)

The Rabbit Polyclonal anti-PLK1 antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect PLK1 in samples from Human, Mouse and Rat.
Catalog No. ABIN744938

Quick Overview for PLK1 antibody (pSer137) (ABIN744938)

Target

See all PLK1 Antibodies
PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity

  • 123
  • 45
  • 38
  • 15
  • 7
  • 7
  • 6
  • 5
  • 5
  • 3
  • 3
  • 3
  • 1
  • 1
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  • 1
Human, Mouse, Rat

Host

  • 98
  • 33
  • 2
  • 2
Rabbit

Clonality

  • 102
  • 33
Polyclonal

Conjugate

  • 77
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PLK1 antibody is un-conjugated

Application

  • 110
  • 48
  • 33
  • 27
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  • 19
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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

    • 21
    • 18
    • 15
    • 12
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer137

    Specificity

    This phosphorylation site is homologous across the listed species.

    Cross-Reactivity

    Human, Mouse, Rat

    Predicted Reactivity

    Dog,Cow,Pig,Chicken,Rabbit

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Expiry Date

    12 months
  • Target

    PLK1 (Polo-Like Kinase 1 (PLK1))

    Alternative Name

    PLK1

    Background

    Synonyms: PLK, STPK13, Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, PLK1

    Background: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Phosphorylates NEDD1. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylates both ECT2 and RACGAP1, and thereby stimulates their interaction that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2.

    Gene ID

    5347

    UniProt

    P53350

    Pathways

    Cell Division Cycle, M Phase
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