MAP2K4 antibody (pSer257, pThr261)

Catalog No. ABIN745673

The Rabbit Polyclonal anti-MAP2K4 antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect MAP2K4 in samples from Human and Mouse.

Quick Overview for MAP2K4 antibody (pSer257, pThr261) (ABIN745673)

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAP2K4 Antibody

Binding Specificity: pSer257, pThr261

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of Ser257/Thr261

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MAP2K4

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Alternative Name: MEK4 +

Background:

Synonyms: MEK4 phospho S257 + T261, SEK1/MKK4Ser257 + Thr261, MAP2K4phospho S257 + T261, MKK4 phospho Ser257 + Thr261, p-MKK4 Ser257 + Thr261, c Jun N terminal kinase kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun N-terminal kinase kinase 1, Dual specicity mitogen activated protein kinase kinase 4, Dual specicity mitogen activated protein kinase kinase 4, Dual specicity mitogen-activated protein kinase kinase 4, JNK activated kinase 1, JNK Activated Kinase 1, JNK activating kinase 1, JNK activating kinase 1, JNK-activating kinase 1, JNKK 1, JNKK, JNKK, JNKK-1, JNKK-1, JNKK1, JNKK1, MAP kinase kinase 4, MAP kinase kinase 4, MAP2K4, MAPK / ERK kinase 4, MAPK / ERK kinase 4, MAPK ERK kinase 4, MAPK/ERK kinase 4, MAPKK 4, MAPKK 4, MAPKK-4, MAPKK-4, MAPKK4, MAPKK4, MEK 4, MEK 4, MEK-4, MEK-4, MEK4, Mitogen Activated Protein Kinase Kinase 4, MKK 4, MKK-4, MKK-4, MKK4, MKK4, MP2K4_HUMAN, PRKMK 4, PRKMK 4, PRKMK-4, PRKMK-4, PRKMK4, PRKMK4, SAPK / ERK kinase 1, SAPK / ERK kinase 1, SAPK ERK kinase 1, SAPK/ERK Kinase 1, SEK 1, SEK1, SEK1, SERK 1, SERK-1, SERK-1, SERK1, SERK1

Background: This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Gene ID: 6416

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling