BRD2 antibody (AA 58-232)

Catalog No. ABIN7938062

The Rabbit Polyclonal anti-BRD2 antibody is suitable to detect BRD2 in samples from Human. It has been validated for ChIP-seq, ChIP and CUT&RUN.

Quick Overview for BRD2 antibody (AA 58-232) (ABIN7938062)

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD2 antibody is un-conjugated

Application: ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Product Details anti-BRD2 Antibody

Binding Specificity: AA 58-232

Purpose: BRD2 antibody (pAb)

Purification: Affinity Purified

Immunogen: This BRD2 antibody was raised against a recombinant protein comprising amino acids 58-232 of human BRD2 (NP_005095 ).

Isotype: IgG

Application Details

Application Notes: ChIP-Seq: 6 µL per ChIP CUT&RUN: 1 µL per 50 µL reaction

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for BRD2

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Alternative Name: BRD2

Background:

Background_Plain: BRD2 (Bromodomain-containing protein 2) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.

Aliases: Bromodomain Containing 2, O27.1.1, RING3

Molecular Weight: 110 kDa

NCBI Accession: NP_005095

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection