Retinoblastoma Binding Protein 8 antibody (AA 620-897)

Catalog No. ABIN7938099

The Mouse Monoclonal anti-Retinoblastoma Binding Protein 8 antibody (Clone 14-1) (ABIN7938099) specifically detects Retinoblastoma Binding Protein 8 in WB, IP, IF, ICC and ChIP. The antibody is reactive with Human samples.

Quick Overview for Retinoblastoma Binding Protein 8 antibody (AA 620-897) (ABIN7938099)

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma Binding Protein 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Clone: 14-1

Product Details anti-Retinoblastoma Binding Protein 8 Antibody

Binding Specificity: AA 620-897

Purpose: CtIP antibody (mAb) (Clone 14-1)

Purification: Protein A Chromatography

Immunogen: This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

Isotype: IgG1

Application Details

Application Notes: WB: 1 - 2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Retinoblastoma Binding Protein 8

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Alternative Name: CtIP

Background:

Background_Plain: CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.

Aliases: RBBP8, RIM, COM1, JWDS, SAE2, SCKL2

Molecular Weight: 120 kDa

NCBI Accession: NP_002885

Pathways: Cell Division Cycle, DNA Damage Repair