Recombinant JNK antibody (pTyr185)

Catalog No. ABIN7938300

The Rabbit Monoclonal anti-JNK antibody (Clone 2G8) (ABIN7938300) specifically detects JNK in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant JNK antibody (pTyr185) (ABIN7938300)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2G8

Product Details anti-JNK Antibody

Binding Specificity: pTyr185

Purpose: JNK phospho Tyr185 (rAb), 100 μg (Clone 2G8)

Purification: Protein A Chromatography

Immunogen: This information is proprietary to Active Motif and/or its suppliers.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:40000 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK

Background: MAPK8 (Mitogen-activated protein kinase 8) is also named JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun.

Molecular Weight: 37-46 kDa

NCBI Accession: NM_139049

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins