Recombinant p300 antibody

Catalog No. ABIN7938388

This Rabbit Monoclonal antibody specifically detects p300 in IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant p300 antibody (ABIN7938388)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This p300 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Clone: 230369D12

Product Details anti-p300 Antibody

Purpose: p300 antibody (rAb), 100 μg (Clone 230369D12)

Purification: Protein A Chromatography

Immunogen: This information is proprietary to Active Motif and/or its suppliers.

Isotype: IgG

Application Details

Application Notes: IP: 4 μg per IP

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: p300

Background:

Background_Plain: p300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.

Aliases: EP300, KAT3B, RSTS2

Molecular Weight: 264 kDa

NCBI Accession: NM_001429

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size