Recombinant Topoisomerase II alpha antibody

Catalog No. ABIN7938529

This Rabbit Monoclonal antibody specifically detects Topoisomerase II alpha in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant Topoisomerase II alpha antibody (ABIN7938529)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1J18

Product Details anti-Topoisomerase II alpha Antibody

Purpose: TOP2A antibody (rAb), 100 μl (Clone 1J18)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against the TOP2A fusion protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:50000 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background:

Background_Plain: TOP2A, also named as TOP2A, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2A control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Aliases: DNA topoisomerase Iia

Molecular Weight: 174 kDa

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases