Actin antibody (C-Term)

Catalog No. ABIN7965692

The Mouse Monoclonal anti-Actin antibody is suitable to detect Actin in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for Actin antibody (C-Term) (ABIN7965692)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: 3H5

Product Details anti-Actin Antibody

Binding Specificity: C-Term

Purpose: Anti-Actin/ACTA1 Antibody (monoclonal, 3H5)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-Actin/ACTA1 Antibody.(monoclonal, 3H5)-5. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background:

Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Gene Full Name: actin alpha 1, skeletal muscle

Molecular Weight: 43 kDa

Gene ID: 58

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development