ANAPC2 antibody (AA 51-272)

Catalog No. ABIN7968365

The Rabbit Polyclonal anti-ANAPC2 antibody is suitable to detect ANAPC2 in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, ELISA, IHC, FACS, IF and ICC.

Quick Overview for ANAPC2 antibody (AA 51-272) (ABIN7968365)

Target: ANAPC2 (Anaphase Promoting Complex Subunit 2 (ANAPC2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-ANAPC2 Antibody

Binding Specificity: AA 51-272

Purpose: Anti-ANAPC2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ANAPC2 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ANAPC2 recombinant protein (Position: K51-R272).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ANAPC2

Target: ANAPC2 (Anaphase Promoting Complex Subunit 2 (ANAPC2))

Alternative Name: ANAPC2

Background:

Background: Anaphase-promoting complex subunit 2 is an enzyme that in humans is encoded by the ANAPC2 gene. A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.

Gene Full Name: anaphase promoting complex subunit 2

Molecular Weight: 110 kDa

Gene ID: 29882

UniProt: Q9UJX6

Pathways: Regulation of Cell Size