Annexin a1 antibody (AA 2-346) (HRP)

Catalog No. ABIN7968834

This Mouse Monoclonal antibody specifically detects Annexin a1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Annexin a1 antibody (AA 2-346) (HRP) (ABIN7968834)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin a1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 6B7F8

Product Details anti-Annexin a1 Antibody (HRP)

Binding Specificity: AA 2-346

Purpose: Anti-Annexin A1/ANXA1 Antibody (monoclonal, 6B7F8) HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Annexin A1 shares 88%,89% amino acid (aa) sequence identity with mouse,rat Annexin A1,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequence identity with mouse and rat Annexin A1, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: ANXA1

Background:

Background: ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Gene Full Name: annexin A1

Gene ID: 301

UniProt: P04083

Pathways: Hormone Transport