CAPNL1 antibody (AA 396-555)

Catalog No. ABIN7974313

The Mouse Monoclonal anti-CAPNL1 antibody is suitable to detect CAPNL1 in samples from Human. It has been validated for WB, ICC, FACS and IF.

Quick Overview for CAPNL1 antibody (AA 396-555) (ABIN7974313)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAPNL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Clone: 2I10

Product Details anti-CAPNL1 Antibody

Binding Specificity: AA 396-555

Purpose: Anti-Calpain 1 Antibody (monoclonal, 2I10)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse,rat Calpain 1.

Characteristics: Anti-Calpain 1 Antibody.(monoclonal, 2I10)-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Alternative Name: CAPN1

Background:

Background: CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Gene Full Name: calpain 1

Molecular Weight: 82 kDa

Gene ID: 823

UniProt: P07384

Pathways: Apoptosis