CAPNL1 antibody (Middle Region) (Cy3)

Catalog No. ABIN7974326

The Cy3-conjugated Rabbit Polyclonal anti-CAPNL1 antibody (ABIN7974326) specifically detects CAPNL1 in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CAPNL1 antibody (Middle Region) (Cy3) (ABIN7974326)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPNL1 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-CAPNL1 Antibody (Cy3)

Binding Specificity: Middle Region

Purpose: Anti-Calpain 1/CAPN1 Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the mouse sequence by two amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1, different from the mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Alternative Name: CAPN1

Background:

Background: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2. By quantitative RT-PCR, Ueyama et al. (1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al. (1989, 1990) assign CANPL1 to chromosome 11.

Gene Full Name: calpain 1

Sequence Similarities: Belongs to the peptidase C2 family.

Gene ID: 823

UniProt: P07384

Pathways: Apoptosis