Caspase 1 antibody (AA 132-404) (Cy3)

Catalog No. ABIN7975130

The Cy3-conjugated Rabbit Polyclonal anti-Caspase 1 antibody (ABIN7975130) specifically detects Caspase 1 in FACS. The antibody is reactive with Human samples.

Quick Overview for Caspase 1 antibody (AA 132-404) (Cy3) (ABIN7975130)

Target: Caspase 1 (CASP1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-Caspase 1 Antibody (Cy3)

Binding Specificity: AA 132-404

Purpose: Anti-Caspase 1(p20)/CASP1 Antibody Cy3 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Caspase 1(p20)/CASP1 recombinant protein (Position: N132-H404).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: CASP1

Background:

Background: Caspase 1, apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. Caspase 1 was purified ICE from the cytosol of the THP. human monocytic cell line and found that the active protease was made up of 2 peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

Gene Full Name: caspase 1

Gene ID: 834

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome