CARD10 antibody (AA 63-1014)

Catalog No. ABIN7975612

The Rabbit Polyclonal anti-CARD10 antibody is suitable to detect CARD10 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and FACS.

Quick Overview for CARD10 antibody (AA 63-1014) (ABIN7975612)

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-CARD10 Antibody

Binding Specificity: AA 63-1014

Purpose: Anti-CARD10 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human CARD10 shares 90.7% amino acid (aa) sequence identity with mouse CARD10.

Characteristics: Anti-CARD10 Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CARD10 recombinant protein (Position: E63-R1014). Human CARD10 shares 90.7% amino acid (aa) sequence identity with mouse CARD10.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CARD10

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Alternative Name: CARD10

Background:

Background: Caspase recruitment domain-containing protein 10 is a protein in the CARD-CC protein family that in humans is encoded by the CARD10 gene. The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B through BCL10.

Gene Full Name: caspase recruitment domain family member 10

Molecular Weight: 116 kDa

Gene ID: 29775

UniProt: Q9BWT7

Pathways: S100 Proteins