Cullin 4B antibody (C-Term) (APC)

Catalog No. ABIN7982050

The Rabbit Polyclonal anti-Cullin 4B antibody is suitable to detect Cullin 4B in samples from Human, Mouse, Rat and Zebrafish (Danio rerio). It has been validated for FACS.

Quick Overview for Cullin 4B antibody (C-Term) (APC) (ABIN7982050)

Target: Cullin 4B (CUL4B)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 4B antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Product Details anti-Cullin 4B Antibody (APC)

Binding Specificity: C-Term

Purpose: Anti-Cullin 4B/CUL4B Antibody APC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Cullin 4B

Target: Cullin 4B (CUL4B)

Alternative Name: CUL4B

Background:

Background: Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B (AhR). Complex assembly and ubiquitin ligase activity of CUL4B (AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B (AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.

Gene Full Name: cullin 4B

Sequence Similarities: Belongs to the cullin family.

Gene ID: 8450

UniProt: Q13620