Cyclin B1 antibody (AA 1-433) (Fluoro488)

Catalog No. ABIN7982123

The Fluoro488-conjugated Rabbit Polyclonal anti-Cyclin B1 antibody (ABIN7982123) specifically detects Cyclin B1 in FACS. The antibody is reactive with Human samples.

Quick Overview for Cyclin B1 antibody (AA 1-433) (Fluoro488) (ABIN7982123)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-Cyclin B1 Antibody (Fluoro488)

Binding Specificity: AA 1-433

Purpose: Anti-Cyclin B1/CCNB1 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Cyclin B1 shares 86%,85% amino acid (aa) sequences identity with mouse,rat Cyclin B1,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB

Background:

Background: CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

Gene Full Name: cyclin B1

Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase