Cyclin D1 antibody (C-Term) (Fluoro488)

Catalog No. ABIN7982167

The Rabbit Polyclonal anti-Cyclin D1 antibody is suitable to detect Cyclin D1 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for Cyclin D1 antibody (C-Term) (Fluoro488) (ABIN7982167)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-Cyclin D1 Antibody (Fluoro488)

Binding Specificity: C-Term

Purpose: Anti-Cyclin D1/CCND1 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat sequence,and different from the related human sequence by three amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1, identical to the related rat sequence, and different from the related human sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Background:

Background: Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL, 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.

Gene Full Name: cyclin D1

Sequence Similarities: Belongs to the cyclin family. Cyclin D subfamily.

Gene ID: 12443

UniProt: P25322

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling