CDK2 antibody (AA 81-298)

Catalog No. ABIN7982307

This Rabbit Polyclonal antibody specifically detects CDK2 in WB, IHC, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for CDK2 antibody (AA 81-298) (ABIN7982307)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-CDK2 Antibody

Binding Specificity: AA 81-298

Purpose: Anti-Cdk2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

Characteristics: Anti-Cdk2 Antibody. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background:

Background: CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2)/cyclin A. The gene for p33 shares 65 % sequence identity with p34 (cdc2). P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Gene Full Name: cyclin dependent kinase 2

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Molecular Weight: 33 kDa

Gene ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA