DDX21 antibody (AA 395-660) (Fluoro488)

Catalog No. ABIN7984043

The Fluoro488-conjugated Rabbit Polyclonal anti-DDX21 antibody (ABIN7984043) specifically detects DDX21 in WB, ELISA, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for DDX21 antibody (AA 395-660) (Fluoro488) (ABIN7984043)

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX21 antibody is conjugated to Fluoro488

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-DDX21 Antibody (Fluoro488)

Binding Specificity: AA 395-660

Purpose: Anti-DDX21 Antibody Fluoro488 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DDX21 recombinant protein (Position: E395-Q660).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for DDX21

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Alternative Name: DDX21

Background:

Background: Nucleolar RNA helicase 2 is an enzyme that in humans is encoded by the DDX21 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.

Gene Full Name: DExD-box helicase 21

Gene ID: 9188

UniProt: Q9NR30

Pathways: SARS-CoV-2 Protein Interactome