DEPTOR antibody (AA 22-409)

Catalog No. ABIN7984919

The Rabbit Polyclonal anti-DEPTOR antibody (ABIN7984919) specifically detects DEPTOR in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DEPTOR antibody (AA 22-409) (ABIN7984919)

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPTOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-DEPTOR Antibody

Binding Specificity: AA 22-409

Purpose: Anti-DEPDC6/DEPTOR Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human DEPDC6,DEPTOR shares 97.9% amino acid (aa) sequence identity with mouse DEPDC6,DEPTOR.

Characteristics: Anti-DEPDC6-DEPTOR Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DEPDC6/DEPTOR recombinant protein (Position: A22-C409). Human DEPDC6/DEPTOR shares 97.9% amino acid (aa) sequence identity with mouse DEPDC6/DEPTOR.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Alternative Name: DEPTOR

Background:

Background: DEP domain-containing mTOR-interacting protein (DEPTOR) also known as DEP domain-containing protein 6 (DEPDC6) is a protein that in humans is encoded by the DEPTOR gene. Enables phosphatidic acid binding activity and protein serine/threonine kinase inhibitor activity. Involved in several processes, including negative regulation of TOR signaling, negative regulation of cell size, and negative regulation of protein kinase activity. Is active in lysosomal membrane.

Gene Full Name: DEP domain containing MTOR interacting protein

Molecular Weight: 48 kDa

Gene ID: 64798

UniProt: Q8TB45

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect