Dihydrofolate Reductase antibody (AA 2-187) (Cy3)

Catalog No. ABIN7985258

The Cy3-conjugated Mouse Monoclonal anti-Dihydrofolate Reductase antibody (Clone 3C8) (ABIN7985258) specifically detects Dihydrofolate Reductase in FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for Dihydrofolate Reductase antibody (AA 2-187) (Cy3) (ABIN7985258)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dihydrofolate Reductase antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Clone: 3C8

Product Details anti-Dihydrofolate Reductase Antibody (Cy3)

Binding Specificity: AA 2-187

Purpose: Anti-DHFR Antibody (monoclonal, 3C8) Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background:

Background: SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: dihydrofolate reductase

Gene ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases