DRAP1 antibody (AA 15-166)

Catalog No. ABIN7986375

This Rabbit Polyclonal antibody specifically detects DRAP1 in ELISA, WB, IHC, FACS, IF, ICC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DRAP1 antibody (AA 15-166) (ABIN7986375)

Target: DRAP1 (DR1-Associated Protein 1 (Negative Cofactor 2 Alpha) (DRAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRAP1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: Carrier-free

Product Details anti-DRAP1 Antibody

Binding Specificity: AA 15-166

Purpose: Anti-DRAP1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DRAP1 Antibody. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DRAP1 recombinant protein (Position: A15-S166).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DRAP1

Target: DRAP1 (DR1-Associated Protein 1 (Negative Cofactor 2 Alpha) (DRAP1))

Alternative Name: DRAP1

Background:

Background: Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex.

Gene Full Name: DR1 associated protein 1

Molecular Weight: 26 kDa

Gene ID: 10589

UniProt: Q14919