EGLN1 antibody (C-Term) (Biotin)

Catalog No. ABIN7987211

The Biotin-conjugated Rabbit Polyclonal anti-EGLN1 antibody (ABIN7987211) specifically detects EGLN1 in WB, IHC and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EGLN1 antibody (C-Term) (Biotin) (ABIN7987211)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-EGLN1 Antibody (Biotin)

Binding Specificity: C-Term

Purpose: Anti-PHD2/EGLN1 Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PHD2,EGLN1 shares 100% amino acid (aa) sequence identity with both mouse,rat PHD2,EGLN1.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PHD2/EGLN1. Human PHD2/EGLN1 shares 100% amino acid (aa) sequence identity with both mouse and rat PHD2/EGLN1.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background:

Background: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).

Gene Full Name: egl-9 family hypoxia inducible factor 1

Gene ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect