ELANE antibody (AA 27-265) (PE)

Catalog No. ABIN7987297

The Rabbit Polyclonal anti-ELANE antibody is suitable to detect ELANE in samples from Mouse and Rat. It has been validated for FACS.

Quick Overview for ELANE antibody (AA 27-265) (PE) (ABIN7987297)

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELANE antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-ELANE Antibody (PE)

Binding Specificity: AA 27-265

Purpose: Anti-Neutrophil Elastase/ELANE Antibody PE Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: coli-derived mouse Elastase,ELA2.,ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase,ELANE,ELA2 recombinant protein (Position: S27-N265). Mouse Elastase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: Elane

Background:

Background: Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Gene Full Name: elastase, neutrophil expressed

Gene ID: 50701

UniProt: Q3UP87