EEF2K antibody (AA 162-719)

Catalog No. ABIN7988588

The Rabbit Polyclonal anti-EEF2K antibody (ABIN7988588) specifically detects EEF2K in WB, ELISA, IF, ICC and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for EEF2K antibody (AA 162-719) (ABIN7988588)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-EEF2K Antibody

Binding Specificity: AA 162-719

Purpose: Anti-EEF2K Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EEF2K Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EEF2K recombinant protein (Position: K162-A719).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: EEF2K

Background:

Background: Eukaryotic elongation factor-2 kinase (eEF-2 kinase or eEF-2K), also known as calmodulin-dependent protein kinase III (CAMKIII) and calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, is an enzyme that in humans is encoded by the EEF2K gene. This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Gene Full Name: eukaryotic elongation factor 2 kinase

Molecular Weight: 82 kDa

Gene ID: 29904

UniProt: O00418

Pathways: AMPK Signaling