Fibronectin 1 antibody (AA 869-2014)

Catalog No. ABIN7990818

This Rabbit Polyclonal antibody specifically detects Fibronectin 1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Fibronectin 1 antibody (AA 869-2014) (ABIN7990818)

Target: Fibronectin 1 (FN1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Fibronectin 1 Antibody

Binding Specificity: AA 869-2014

Purpose: Anti-Fibronectin/FN1 Antibody

Predicted Reactivity: Human FN1 shares 91.2%,91.4% amino acid (aa) sequence identity with mouse,rat FN1,respectively.

Characteristics: Anti-Fibronectin/FN1 Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Fibronectin/FN1 recombinant protein (Position: D869-W2014). Human FN1 shares 91.2% and 91.4% amino acid (aa) sequence identity with mouse and rat FN1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1

Background:

Background: Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. It is mapped to 2q35. This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.

Gene Full Name: fibronectin 1

Molecular Weight: 272 kDa

Gene ID: 2335

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy