Connexin 43/GJA1 antibody (C-Term)

Catalog No. ABIN7992639

This Rabbit Polyclonal antibody specifically detects Connexin 43/GJA1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Connexin 43/GJA1 antibody (C-Term) (ABIN7992639)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: C-Term

Purpose: Anti-Connexin 43/GJA1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-Connexin 43/GJA1 Antibody-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Frozen Section), 0.5-1 μg/mLImmunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mLWestern blot, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1

Background:

Background: Connexins 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Gene Full Name: gap junction protein alpha 1

Sequence Similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.

Molecular Weight: 43 kDa

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization