GRIA2 antibody (AA 25-360)

Catalog No. ABIN7993696

The Rabbit Polyclonal anti-GRIA2 antibody is suitable to detect GRIA2 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ICC, IF, FACS and IHC (fro).

Quick Overview for GRIA2 antibody (AA 25-360) (ABIN7993696)

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: Carrier-free

Product Details anti-GRIA2 Antibody

Binding Specificity: AA 25-360

Purpose: Anti-Ionotropic Glutamate receptor 2/GRIA2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse,rat GRIA2.

Characteristics: Anti-Ionotropic Glutamate receptor 2/GRIA2 Antibody. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRIA2 recombinant protein (Position: N25-I360). Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat Immunocytochemistry, 0.5-1 μg/mL, Human Immunofluorescence, 2 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Alternative Name: GRIA2

Background:

Background: Glutamate receptor 2, also known as GLUR2, is a protein that in humans is encoded by the GRIA2 gene. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. GLUR2's cytogenetic location is 4q32.1. The crystal structures of the GLUR2 ligand-binding core in the apo state and in the presence of the antagonist DNQX, the partial agonist kainate, and the full agonists AMPA and glutamate.GLUR2 plays a major role in depression at synapses in which glutamate remains in the synaptic cleft for prolonged periods of time during normal operation of the synapse. The overexpression of GLUR2 increases dendritic spine size and density in hippocampal neurons, and more remarkably, induces spine formation in GABA-releasing interneurons that normally lack spines.

Gene Full Name: glutamate ionotropic receptor AMPA type subunit 2

Molecular Weight: 110 kDa

Gene ID: 14800

UniProt: P42262

Pathways: PI3K-Akt Signaling