Golgin B1 (GOLGB1) (AA 153-950) antibody

Catalog No. ABIN7994986

The Rabbit Polyclonal anti-Golgin B1 antibody is suitable to detect Golgin B1 in samples from Human. It has been validated for WB, FACS, ELISA, IHC, ICC and IF.

Quick Overview for Golgin B1 (GOLGB1) (AA 153-950) antibody (ABIN7994986)

Target: Golgin B1 (GOLGB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details

Binding Specificity: AA 153-950

Purpose: Anti-Giantin/GOLGB1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Giantin/GOLGB1 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Giantin/GOLGB1 recombinant protein (Position: L153-E950).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.5-1 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Golgin B1 (GOLGB1)

Alternative Name: GOLGB1

Background:

Background: Giantin or Golgin subfamily B member 1 is a protein that in humans is encoded by the GOLGB1 gene. GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family. GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton. It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis. GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome. Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92 % and 91 % sequence identity with its mouse and rat orthologs, respectively.

Gene Full Name: golgin B1

Molecular Weight: 376 kDa

Gene ID: 2804

UniProt: Q14789

Pathways: SARS-CoV-2 Protein Interactome