Hexokinase 2 antibody (Middle Region) (Biotin)

Catalog No. ABIN7997431

The Rabbit Polyclonal anti-Hexokinase 2 antibody is suitable to detect Hexokinase 2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for Hexokinase 2 antibody (Middle Region) (Biotin) (ABIN7997431)

Target: Hexokinase 2 (HK2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Hexokinase 2 Antibody (Biotin)

Binding Specificity: Middle Region

Purpose: Anti-Hexokinase II/HK2 Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse,rat sequences by four amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Hexokinase II, different from the related mouse and rat sequences by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HK2

Background:

Background: Hexokinase 2, also known as HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Gene Full Name: hexokinase 2

Gene ID: 3099

UniProt: P52789

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect