HSD17B1 antibody

Catalog No. ABIN7998751

The Rabbit Polyclonal anti-HSD17B1 antibody is suitable to detect HSD17B1 in samples from Human. It has been validated for WB, IHC, ELISA, ICC and IF.

Quick Overview for HSD17B1 antibody (ABIN7998751)

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-HSD17B1 Antibody

Purpose: Anti-HSD17B1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HSD17B1 Antibody. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human HSD17B1 recombinant protein (A20-K249).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HSD17B1

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Alternative Name: HSD17B1

Background:

Background: Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

Gene Full Name: hydroxysteroid 17-beta dehydrogenase 1

Molecular Weight: 40 kDa

Gene ID: 3292

UniProt: P14061

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis