MCL-1 antibody (AA 1-350)

Catalog No. ABIN7999379

The Rabbit Polyclonal anti-MCL-1 antibody (ABIN7999379) specifically detects MCL-1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MCL-1 antibody (AA 1-350) (ABIN7999379)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-MCL-1 Antibody

Binding Specificity: AA 1-350

Purpose: Anti-MCL1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human MCL1 shares 82%,78% amino acid (aa) sequences identity with mouse,rat MCL1,respectively.

Characteristics: Anti-MCL1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, RatWestern blot, 0.1-0.5 μg/mL, Human, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1

Background:

Background: MCL1, also known as myeloid cell leukemia 1, is a protein that in humans is encoded the MCL1 gene. It is mapped to 1q21.3. MCL1 is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. MCL1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of MCL1 s its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.

Gene Full Name: MCL1 apoptosis regulator, BCL2 family member

Sequence Similarities: Belongs to the Bcl-2 family.

Molecular Weight: 37 kDa

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling