INSIG1 antibody (N-Term) (Fluoro647)

Catalog No. ABIN7999821

The Rabbit Polyclonal anti-INSIG1 antibody is suitable to detect INSIG1 in samples from Mouse and Rat. It has been validated for FACS.

Quick Overview for INSIG1 antibody (N-Term) (Fluoro647) (ABIN7999821)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-INSIG1 Antibody (Fluoro647)

Binding Specificity: N-Term

Purpose: Anti-Insig1 Antibody Fluoro647 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat Insig1, which shares 71.4% and 92.9% amino acid (aa) sequence identity with human and mouse Insig1, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: Insig1

Background:

Background: Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.

Gene Full Name: insulin induced gene 1

Gene ID: 64194

UniProt: Q08755

Pathways: ER-Nucleus Signaling