IGF1R antibody (AA 44-960) (Biotin)

Catalog No. ABIN7999977

The Rabbit Polyclonal anti-IGF1R antibody is suitable to detect IGF1R in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for IGF1R antibody (AA 44-960) (Biotin) (ABIN7999977)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1R antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IGF1R Antibody (Biotin)

Binding Specificity: AA 44-960

Purpose: Anti-IGF1R Antibody Biotin Conjugated

Predicted Reactivity: Human IGF1R shares 95.9%,95.8% amino acid (aa) sequence identity with mouse,rat IGF1R,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IGF1R recombinant protein (Position: Q44-R960). Human IGF1R shares 95.9% and 95.8% amino acid (aa) sequence identity with mouse and rat IGF1R, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R

Background:

Background: IGF1R(Insulin-like Growth Factor 1(IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1(IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al.(1998) identified a regulatory subunit of phosphatidylinositol(PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45 % of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.

Gene Full Name: insulin like growth factor 1 receptor

Gene ID: 3480

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy