ILF2 antibody (AA 88-1165)

Catalog No. ABIN8002903

The Rabbit Polyclonal anti-ILF2 antibody (ABIN8002903) specifically detects ILF2 in WB, ELISA, IHC, FACS, IF and ICC. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for ILF2 antibody (AA 88-1165) (ABIN8002903)

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-ILF2 Antibody

Binding Specificity: AA 88-1165

Purpose: Anti-NF45/ILF2 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PC shares 97%,96.6% amino acid (aa) sequence identity with mouse,rat PC,respectively.

Characteristics: Anti-NF45/ILF2 Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Pyruvate Carboxylase/PC recombinant protein (Position: R88-D1165). Human PC shares 97% and 96.6% amino acid (aa) sequence identity with mouse and rat PC, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ILF2

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Alternative Name: ILF2

Background:

Background: Interleukin enhancer-binding factor 2 is a protein that in humans is encoded by the ILF2 gene. The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.

Gene Full Name: interleukin enhancer binding factor 2

Molecular Weight: 43 kDa

Gene ID: 3608

UniProt: Q12905