ITCH antibody (AA 17-358) (Cy3)

Catalog No. ABIN8003532

The Rabbit Polyclonal anti-ITCH antibody is suitable to detect ITCH in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for ITCH antibody (AA 17-358) (Cy3) (ABIN8003532)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-ITCH Antibody (Cy3)

Binding Specificity: AA 17-358

Purpose: Anti-ITCH/AIP4 Antibody Cy3 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background:

Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: itchy E3 ubiquitin protein ligase

Gene ID: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events