KLF8 antibody (N-Term) (Biotin)

Catalog No. ABIN8004775

The Biotin-conjugated Rabbit Polyclonal anti-KLF8 antibody (ABIN8004775) specifically detects KLF8 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for KLF8 antibody (N-Term) (Biotin) (ABIN8004775)

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF8 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-KLF8 Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Anti-Krueppel-like factor 8 KLF8 Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for KLF8

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Alternative Name: KLF8

Background:

Background: KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614). Suske et al. (2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al. (2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.

Gene Full Name: KLF transcription factor 8

Sequence Similarities: Belongs to the Sp1 C2H2-type zinc-finger protein family.

Gene ID: 11279

UniProt: O95600