Lamin A/C antibody (AA 481-646) (Fluoro594)

Catalog No. ABIN8004977

This Mouse Monoclonal antibody specifically detects Lamin A/C in FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Lamin A/C antibody (AA 481-646) (Fluoro594) (ABIN8004977)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is conjugated to Fluoro594

Application: Flow Cytometry (FACS)

Clone: 5F3C12

Product Details anti-Lamin A/C Antibody (Fluoro594)

Binding Specificity: AA 481-646

Purpose: Anti-Lamin A+C/LMNA Antibody (monoclonal, 5F3C12) Fluoro594 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Lamin A,C shares 90%,92% amino acid (aa) sequence identity with mouse,rat Lamin A,C,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: LMNA

Background:

Background: Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Gene Full Name: lamin A/C

Gene ID: 4000

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus